Title The Effect of Different Culture Media on Production of Phaeodactylum tricornutum
Abstract Phaeodactylum tricornutum is a marine, unicellular diatom. This microalga can exist in different morphotypes (fusiform, triradiate, and oval), and changes in cell shape can be stimulated by environmental conditions. Furthermore, P. tricornutum is the second diatom for which a whole genome sequence has been generated. In addition to protein, carbohydrate and lipid content, fucoxanthin pigment is one of the important factors for the production and usage of P.tricornutum commercially. The main objective of the current study, was to investigate the effect of component deficiency (nitrogen free and phosphate free F/2 media) in F/2 culture medium on the production of fucoxanthin by P. tricornutum. With this aim, the cells were cultivated in five different culture media as the nitrogen free, phosphate free, nitrogen-phosphate free and normal F/2 culture media at 24 °C with the air flow rate of 3 vvm during 13 days of the cultivation period. It was recorded that, maximum specific growth rate of 0.2137 day-1 was obtained in the nitrogen free medium at 24 °C with the air flow rate of 3 vvm.. However, when the fucoxanthin production was considered, the maximum product yield of 3.01 mg. L-1.day-1 was found in F/2 culture medium. Important point to note here is that fucoxanthin content reduced under the condition of nutrient starvation. Consequently, fucoxanthin content, as a primer metabolite, increased with increasing the growth rate, and the application of stress conditions reduced the cell growth and fucoxanthin content.
Keywords diatom, fucoxanthin, microalgae, Phaeodactylum tricornutum, pigment
Journal BIBAD - Research Journal Of Biological Sciences
Issue Issue 1
Page 040-042
Year 2016
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