Title Effect of Some Physiological Conditions on Chromohalobacter sp. İsolate's δ-Aminolevulinic Acide Dehydratase (ALAD)
Authors Safiye Elif KORCAN, Sevim Feyza ERDOĞMUŞ, Gökçe BULUT, İbrahim Hakkı CİĞERCİ, Kıymet GÜVEN
Abstract Saline environments are frequently contaminated with heavy metals as a result of industrial or agricultural activities. Heavy-metal pollution represents an important environmental problem due to the toxic effects and their accumulation throughout the food chain leads to serious ecological and health problems. Lead (Pb) is one of the most widely used metals in industries and exposure to Pb continues to be a common problem almost in all over the world. δ-Aminolevulinic acide dehydratase (ALAD; E.C. 4.2.1.24) is a metalloprotein and plays a crucial role in heme synthesis. The inhibition of the enzyme δ-aminolevulinic acid dehydratase is recognized as a useful biomarker of Pb exposure and effect, both in humans and other animal species. Selective inhibition of ALAD with lead is an important biological marker of chemical contamination. In this study the effects of some heavy metals on ALADs activity of Chromohalobacter sp. isolates has been studied in order to determine whether their ALADs could be used as biosensor for lead and other heavy metals contamination. Spektrofotometric method was used to determine ALADs activity. According to the results, Pb negatively effected ALADs activity of Chromohalobacter sp. isolates. Some heavy metals inhibited its activity ratio of Pb(NO3)2 %15, ZnSO4 %38, MnCl2 %54, CoCl2 %36, FeSO4 %15, Ni(NO3)2 %52. Surprisingly, MnSO4 increased its activity up to 8%. ALAD activity was observed at pH between 3 and 11, optimum pH was found to be 5 and it was observed that ALAD has an optimal temperature between 20 and 37 °C.
Keywords ALAD, Chromohalobacter sp., heavy metal, lead (Pb)
Journal BIBAD - Research Journal of Biological Sciences
Issue Issue 1
Page 026-028
Year 2015
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