Title Isolation of 2,4-Dichlorophenoxyacetic Acid-Degrading (2,4-D) Anoxygenic Photosynthetic Bacteria
Authors Hale KÖKSOY, Gönül DÖNMEZ
Abstract The samples used in this study were obtained from Ankara Stream, Beyşehir Lake, the active mud of a paper factory and the active mud of a factory producing 2,4-Dichlorophenoxyacetic acid (2,4-D). These samples were inoculated into AT medium without acetate, including different concentrations of 2,4-D, by “Agar Shake Tube” dilution technique. Inoculated tubes were then incubated in room temperature under permanent light for fifteen days. Single colonies that proliferate in these tubes were passaged into liquid AT medium. The cultures that proliferate in the liquid medium were then purified by the same method. Finally, a total of twelve isolates could proliferate in the AT medium including the highest concentration of 2,4-D (0,1-0,2 gr/l). Five of these isolates were sampled from the active mud of the herbicide factory, four were sampled from Ankara Stream, two were sampled from Beyşehir Lake, and one was sampled from the active mud of the paper factory. When the isolates were defined according to their morphological and biochemical properties, all of them showed the characteristics of Rhodopseudomonas palustris (R. palustris) species. The effects of pH and 2,4-D concentrations on the proliferation of R. palustris strains were determined. It was observed that some of the strains could proliferate in 3,3 gr/l 2,4-D concentration. Also, it was determined that the R. palustris strains used in this study could use 2,4-D at pH 6,5, 7,5, 8,5 and 9,5.
Keywords 2,4-Dichlorophenoxyacetic acid (2,4-D), Rhodopseudomonas palustris, and Isolation.
Journal BIBAD - Research Journal of Biological Sciences
Issue Issue 2
Page 095-100
Year 2012
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