Title The Lipase Production of The Gram Positive Bacteria From Soil and Milk Origin
Authors Nurdan SARAÇ, Rukiye BORAN, Gülten ÖKMEN, Aysel UĞUR
Abstract The bacteria used in this study, with lipolytic activity, were isolated from the soils, collected from around the olive orchards and olive oil factory, and from raw and pasteurized milk, using Tributyrin Agar media. The Gram reactions of the isolates were determined and the gram positive isolates were selected and identifi ed to determine the lipase activity. In addition the Tributyrin Agar media, the lipolytic activity of the strains were determined under visible and U.V. light using Rhodamine-B Agar media. The quantitative lipase activity of all gram positive bacteria were determined with the spectrophotometric method using p- nitrophenyl- palmitate as a substrate. In 9 isolates from soils did not show lipase activity. The lipase activity of the other isolates were determined between 2 U/ml and 581.5 U/ml. The maximum lipase activity showed in Staphylococcus sp. NS 02-1. In 7 isolates from milk did not show lipase activity. The lipase activity of the other isolates were determined between 1.6 U/ml and 564.6 U/ml. The maximum lipase activity showed in Staphylococcus sp. RB 07-2.
Keywords Lipase, bacteria, milk, soil.
Journal BIBAD - Research Journal of Biological Sciences
Issue Issue 2
Page 23-28
Year 2008
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