Title In vitro Plant Regeneration Influence by BAP and IBA in Acinos rotundifolius Pers.
Authors Fethi Ahmet ÖZDEMİR, Murat KURŞAT
Abstract Acinos rotundifolius Pers. is used for medicinal purposes plant. In order to propagate Acinos rotundifolius Pers. in vitro, cotyledon node explants were cultured in MS medium containing different combinations of BAP and IBA supplemented with 3% sucrose and solidified with 0.8% agar. The results showed a significant interaction between BAP and IBA combinations for callus formation percentage, shoot and root regeneration, number of shoots and roots per explant, shoot and root length. Maximum shoot regeneration was noted in MS medium containing 0.50 mg/L BAP. Maximum callus formation was determined in MS medium supplemented with 0.25 mg/L BAP + 2 mg/L IBA. The regenerated shoots were rooted on MS medium containing 0.5 mg/L NAA.
Keywords BAP, IBA, Acinos rotundifolius Pers., Micropropagation
Journal TABAD - Research Journal of Agricultural Sciences
Issue Issue 1
Page 015-018
Year 2014
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