Title Karyotype Features of Berseem Clover (T. alexandrinum L.) and Persian Clover (T. resupinatum L.) Species Which Cultivated in Turkey
Authors Serdar DİRİHAN, Eissa ZARIFI, Derya GÜLOĞLU, Cafer S. SEVİMAY
Abstract Karyomorphological studies were carried out on two putative and cultivated Trifolium species in Turkey; berseem (Trifolium alexandrinum L.) and persian clover (T. resupinatum L.), these two species extensively for their adaptation to different ecological conditions are cultivated as forage plant in Turkey. Meristematic cells of root tips (1 cm in length) were obtained from germinated seeds in petri dishes and pretreated in 1% ά- Bromonaphthalene and 8-Hydroxyquinoline solutions and stained in aceto iron hematoxyline with macerating stained roots by means of the enzyme cytase. The results showed that the Somatic chromosomes of these species are contains equal diploid chromosome number 2n=2x= 16; Avarage chromosome length were 3.45± 0.06 and 4.19± 0.13 for berseem and persian clover, respectively. A phenomenon having satelite were found for four chromosome of berseem clover which was reported undefinite in previous studies; chromosome 1 and chromosome 7 were found submetacentric with the arm ratio 3.26± 0.37 and metacentric with the arm ratio 1.23± 0.10, respectively. Sattelite lentghs of these chromosomes are 1.51 and 0.95 μm. In persian clover chromosomes one pair was found having heteromorphism. This phenomenon was determined in karyotype that as the homologous of chromosome 2, 2(1) was metacentric with the arm ratio 1.16± 0.10 and 2(2) was submetacentric with the arm ratio 2.17± 0.52.
Keywords Trifolium alexandrinum L., Trifolium resupinatum L., heteromorphic chromosome, karyotype
Journal TABAD - Research Journal of Agricultural Sciences
Issue Issue 1
Page 049-054
Year 2014
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