Title The Effect of Water Deficiency Originated Stress in Tissue on Shoot Regeneration of Flax (Linum usitatissimum l.) Hypocotyl Eksplants
Abstract Flax (Linum usitatissimum L.) cv. 'Clarck' seeds, obtained from 'Northern Crop Science Laboratories', in North Dakota, USA were used in the study. Seeds were surface sterilized with 40% commercial bleach containing 5% NaOCl with continuous stirring for 12 min. and then were washed 3-4 times with sterile distilled water. Sterilized seeds were sown in Magenta vessels to germinate and cultured at 24 1ºC with a 16-h light/8-h dark photo-period. Hypocotyl segments of 10-day-old sterile seedlings were excised 0.5 cm in length and then cultured on Murashige and Skoog (MS) medium containing 1 mg l-1 6-benzylaminopurine (BAP) ve 0.02 mg l-1 naphthalene acetic acid (NAA) after two different pretreatment applications. In the first treatment, 0.5 cm hypocotyl explants were directly transferred to regeneration medium while in the other treatment, before culturing, explants were kept in sterile cabin under air flow for 30 min. Four weeks after culture initiation, pretreatments were compared with respect to regeneration percentage, shoot number per explant, the highest shoot length per explant, total shoot number per petri dish, explant fresh and dry weights, explant water and dry matter contents. This study was conducted to determine the effect of air flow in sterile cabin on explants’ regeneration capacity. The highest results in our study were obtained from the treatment in which hypocotyl explants were direcly transferred to regeneration medium. On the other hand, keeping explants under air flow in sterile cabin decreased the regeneration capacity of explants by causing water deficiency in tissue. That is why, when working in sterile cabin, in order to protect the regeneration capacity, explants are to be isolated and placed on growth medium as quickly as possible by considering that air flow in the environment may have negative effect on tissue.
Keywords In vitro shoot regeneration, Linum usitatissimum, tissue water deficiency
Journal TABAD - Research Journal of Agricultural Sciences
Issue Issue 1
Page 153-156
Year 2012
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